The current aim of this research work includes: to evaluate acute toxicity of crude methanol extract of dried ripe fruits of Neoalsomitra clavigera in female albino rats, fractionation of crude methanol extract by normal phase column chromatography, quantification of lupeol by HPTLC in chloroform green band fraction, calculation of LD50 for lupeol rich green column band fraction and their comparative Streptozotocin (STZ) induced in-vivo anti-diabetic activity. The main aim of selection of this research work is to explore leading novel bioactive compound present in ripe fruits of Neoalsomitra clavigera, which has possible role in curing STZ induced diabetes in albino rats. In HPTLC studies, lupeol was quantified (279.43 �µg) in chloroform green band fraction. The lupeol rich chloroform fraction was significantly reduced blood glucose label (p<0.05) on 7th day and significant change found in biochemical parameters like albumin (p<0.05) and cholesterol (p<0.01) on 14th day of drug treatment. a proposed mechanism of antidiabetic action of Neoalsomitra clavigera extract is also given in this research manuscript.\r\nKey words: Neoalsomitra, HPTLC quantification, Lupeol, Anti-diabetic studies.
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